computer assisted sperm analysis (casa) nis-elements Search Results


3d sim image reconstruction  (Nikon)
99
Nikon 3d sim image reconstruction
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
3d Sim Image Reconstruction, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis-elements  (Evident Corporation)
90
Evident Corporation nis-elements
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis elements  (Carl Zeiss)
90
Carl Zeiss nis elements
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis elements software  (QImaging)
90
QImaging nis elements software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements Software, supplied by QImaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis elements ar software  (Nikon)
99
Nikon nis elements ar software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements Ar Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis elements software  (Nikon)
96
Nikon nis elements software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements Software, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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graphpad prism 9  (GraphPad Software Inc)
90
GraphPad Software Inc graphpad prism 9
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Graphpad Prism 9, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lsm 710 confocal microscope  (Carl Zeiss)
90
Carl Zeiss lsm 710 confocal microscope
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Lsm 710 Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c13440-20cu orca-flash4.0 v3 digital cmos camera  (Hamamatsu)
90
Hamamatsu c13440-20cu orca-flash4.0 v3 digital cmos camera
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
C13440 20cu Orca Flash4.0 V3 Digital Cmos Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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metamorph software  (MetaMorph Inc)
90
MetaMorph Inc metamorph software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis-elements imaging software  (Hamamatsu)
90
Hamamatsu nis-elements imaging software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements Imaging Software, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis elements br software  (Canon inc)
90
Canon inc nis elements br software
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
Nis Elements Br Software, supplied by Canon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="100%" height="100%">

Journal: mBio

Article Title: The Polar Organizing Protein PopZ Is Fundamental for Proper Cell Division and Segregation of Cellular Content in Magnetospirillum gryphiswaldense

doi: 10.1128/mBio.02716-18

Figure Lengend Snippet: Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm.

Article Snippet: 3D-SIM image reconstruction was performed in NIS-Elements 5.01 (Nikon) using the “stack reconstruction” algorithm with the following parameter settings.

Techniques: Time-lapse Microscopy, Expressing, Fluorescence, Microscopy, Epifluorescence Microscopy, Staining